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Example Chromatograms
 

 
  Cytochrom C (tryptic digested)  
 
 

In the test below, OPH® RP-L21 column is compared with other leading polystyrene reversed phase HPLC column. Improved column efficiency and peak shape for tailing compounds on OPH® RP-L21 column lead to better chromatography for more accurate and sensitive methods.

 
Cytochrom C (tryptic digested)

 Next Result
     OPH® RP-L21 column
150 mm x 4.6 mm
 
     Leading polystyrene column
(H company)
150 mm x 4.1 mm

Separation Conditions
Mobile phases mobile phases A: 10/90/0.1 ACN/H2O/TFA     B: 90/10/0.1 ACN/H2O/TFA
gradient at 0-15-20-22-25-25.5 min, 100-70-50-10-10-100 A%
Flow 1.5 mL/min
Injection 20 µL
Detection UV 210 nm
Temperature room temperature
Retention time minute
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