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Example Chromatograms
Acetophenone
Amoxicilin
Ampicillin
Benzoic acid
Carbinoxmaine
Cephalexin
Cephradine
Chlorpheniramine
Codeine
Codeine
Cytochrom C (tryptic)
Cytochrom C
Dextromethorphan
Dextromethorphan
Diazepam
Dihydralazine
Diltiazem
Diphenhydramine (Cap)
Diphenhydramine (Gel)
Ephedrine
Furomside
Hydrochlorothiazide
Lysozyme
Methoxy benzylamine
Methyl benzoate
Methyl paraben
Myglobin (Tryptic)
Myglobin
Naphthalene
Norfloxacin
Nucotus expectorant
Pain killer
Parabens
Peptides
Phenoylpropanolamine
Phenylpropanolamine
Promethazine
Promethazine
Promethizaine
Propyl paraben
Pseudoephedrine
Pseudoephedrine
Pyrimidine
Ribonuclease
Sulfadiazine
Sulfamethoxazole
Tetrahydrozoline
Toluene
 

 
  Peptides Mixture (Protein Fragements by Enzymic Hydrolysis)  
 
 

In the test below, OPH® RP-L21 column is compared with other leading polystyrene reversed phase HPLC column. Improved column efficiency and peak shape for tailing compounds on OPH® RP-L21 column lead to better chromatography for more accurate and sensitive methods.

 
 
  Peptides Mixture
(Protein Fragements by Enzymic Hydrolysis)

 
     
Separation Conditions
Column dimension 150 x 4.6 mm
Sample Peptides Mixture
Mobile phases mobile phases A: 5/95/0.1 ACN/H2O/TFA     B: 95/5/0.1 ACN/H2O/TFA
gradient at 0-10-15-20-25 min, 90-70-70-70-90 A%
Flow 1.0 mL/min
Injection 25 µL
Detection UV 215 nm
Temperature room temperature
Retention time minute
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