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In the test below, OPH® RP-L21 column is compared with other leading polystyrene reversed phase HPLC column. Improved column efficiency and peak shape for tailing compounds on OPH® RP-L21 column lead to better chromatography for more accurate and sensitive methods.
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| Ribonuclease, cytochrom C, lysozyme, myglobin |
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Separation Conditions |
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Column dimension |
150 x 4.6 mm |
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Sample |
Protein mixture (Ribonuclease, cytochrom C, lysozyme, myglobin) |
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Mobile phases |
mobile phases A: 5/95/0.1 ACN/H2O/TFA B: 95/5/0.1 ACN/H2O/TFA
gradient at 0-22-27-27.5 min, 85-40-40-85 A% |
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Flow |
1.0 mL/min |
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Injection |
25 µL |
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Detection |
UV 215 nm |
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Temperature |
room temperature |
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Retention time |
minute |
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