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Example Chromatograms
Acetophenone
Amoxicilin
Ampicillin
Benzoic acid
Carbinoxmaine
Cephalexin
Cephradine
Chlorpheniramine
Codeine
Codeine
Cytochrom C (tryptic)
Cytochrom C
Dextromethorphan
Dextromethorphan
Diazepam
Dihydralazine
Diltiazem
Diphenhydramine (Cap)
Diphenhydramine (Gel)
Ephedrine
Furomside
Hydrochlorothiazide
Lysozyme
Methoxy benzylamine
Methyl benzoate
Methyl paraben
Myglobin (Tryptic)
Myglobin
Naphthalene
Norfloxacin
Nucotus expectorant
Pain killer
Parabens
Peptides
Phenoylpropanolamine
Phenylpropanolamine
Promethazine
Promethazine
Promethizaine
Propyl paraben
Pseudoephedrine
Pseudoephedrine
Pyrimidine
Ribonuclease
Sulfadiazine
Sulfamethoxazole
Tetrahydrozoline
Toluene
 

 
  USP L21 polymeric HPLC Columns (Polystyrene-Divinylbenzene based) from Organicphase  
 
 

The following chromatograms are comparison of peptide separation on OPH® RP-L21 column vs leading polystyrene column. There is clear efficiency and resolution improvement in the OPH® RP-L21.


     
  OPH® RP-L21 column   Leading polystyrene column

Column dimension 150 x 4.6 mm
Sample tryptic digest of cytochrome C
Mobile phases mobile phases A: 10/90/0.1 ACN/H2O/TFA     B: 90/10/0.1 ACN/H2O/TFA
gradient at 0-15-20-22-25-25.2 min, 100-70-50-10-10-100 A%
Flow 1.5 mL/min
Injection 25 µL
Detection UV 210 nm
Temperature room temperature
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